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1

Influence of methyl jasmonate on ginsenoside biosynthesis in suspension cultures of Panax quinquefolium L.

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Kochan E., Caban S., Szymańska G., Szymczyk P., Lipert A., Kwiatkowski P., Sienkiewicz M.

Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia

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2017

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vol. 72

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issue 1

EN

Panax quinquefolium L., belonging to the Araliaceae family, along with P. ginseng is one of the well-known species of ginseng. Multidirectional pharmacological action of this plant is attributed to triterpene saponins called ginsenosides. Pharmacopoeial raw material are roots obtained from the field crops which are time-consuming and require expensive agrotechnical procedures. Therefore, the new sources of ginseng biomass are sought such as in vitro suspension cultures. P. quinquefolium L. cell cultures, treated with the elicitation of methyl jasmonate (MJ) in concentration 50 and 250 μmol L-1, synthesize more ginsenosides than control cultures. The highest increase (2.2-fold) of all examined compounds was noted using 250 μmol L-1 MJ. In this condition, the predominantly quantitative metabolite was Rb1 ginsenoside belonging to protopanaxadiol derivatives.

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Ginsenoside content in suspension cultures of Panax quinquefolium L. cultivated in shake flasksand stirred-tank bioreactor

100%

Kochan E., Caban S., Szymańska G., Szymczyk P., Lipert A., Kwiatkowski P., Sienkiewicz M.

Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia

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2017

|

vol. 72

|

issue 1

EN

Plant suspension cultures are described as a source for the acquisition of medicinal secondary metabolites which in the future may become an alternative to traditional raw materials. This study demonstrates that the cell cultures of one of the ginseng species – Panax quinquefolium L. synthesize ginsenosides, which are triterpene saponins having a multidirectional pharmacological effects. Tested suspension cultures were run on a small scale in the shake flasksand in scale up of the process in a 10-liter stirred tank. In the shake flasks,the highest biomass yield (2.28 gl-1 for dry and 33.99 gl-1 for fresh weight) was reached on day 30 of culture, and the highest content of saponins (2.66 mg g -1 dw) was determined on day 28 of culture. In the bioreactor, nearly 2.67 and 3-fold increase of respectively dry and fresh biomass was recorded in relation to the inoculum. Large-scale cultures synthesized protopanaxatriol derivatives such as Rg1 and Re ginsenosides, however, no saponins belonging to the protopanaxadiol derivatives were reported.

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Physical activity of future health care professionals: adherence to current recommendations

100%

Lipert A., Matusiak-Wieczorek E., Kochan E., Szymczyk P., Wrzesińska M., Jegier A.

Medycyna Pracy

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2020

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vol. 71

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issue 5

539-549

EN

BackgroundWhen assessing physical activity (PA), particular attention should be paid to medical university students who are taught to be health care professionals (HCPs) responsible for maintaining health in humans. However, different studies have shown that HCPs exhibit the same unhealthy behaviors as the general population. This study analyzed PA among medical university students of different faculties and their adherence to current PA recommendations.Material and MethodsData from 216 medical university students of physiotherapy, dietetics and pharmacy, including males (N = 44) and females (N = 172), the mean age of 22.3±1.8 years, were collected. The International Physical Activity Questionnaire in its long form (IPAQ-LF) was used to assess and classify PA behaviors. The results were analyzed in accordance with World Health Organization recommendations regarding PA.ResultsOver 60% of all the students were classified as active during all-day activity. However, while analyzing PA in different domains, the same shares of all the students were still insufficiently active during leisure time, and so they did not meet the recommendation of >75 min/week of vigorous PA, >150 min/week of moderate PA or an equivalent combination. All the students self-reported PA mainly in the work and transport domains. Generally, physiotherapy students were the most active and performed PA with higher intensity.ConclusionsThis study revealed a low level of leisure time PA among the students, and no habit of regular PA. Some changes in medical education should be suggested to include physical education as a long-term subject in medical school curricula. Future research is needed to investigate the exercise barriers that students perceive, which can guide future interventions aimed at improving their PA, and thereby impact on the quality of health care which they will provide.

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2 Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia

1 Medycyna Pracy

3 Kochan E.

3 Lipert A.

3 Szymczyk P.

2 Caban S.

2 Kwiatkowski P.

2 Sienkiewicz M.

2 Szymańska G.

1 Jegier A.

1 Matusiak-Wieczorek E.

1 Wrzesińska M.

1 2020

2 2017

Influence of methyl jasmonate on ginsenoside biosynthesis in suspension cultures of Panax quinquefolium L.

Ginsenoside content in suspension cultures of Panax quinquefolium L. cultivated in shake flasksand stirred-tank bioreactor

Physical activity of future health care professionals: adherence to current recommendations