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Aurochs (Bos primigenius) was a unique species lasting through Pleistocene and Holocene for about two million years. The aurochs was one of the largest animals ever to inhabit Europe. The longest the species survived in the very Central Europe (presently Poland), where in spite of being taken under quite modern protection the last aged cow died in the year 1627. The most often brought up reason for dying out of the Aurochs is hunting and pouching. Another belief is, that growing agriculture pushed them away further from human sites. The study of the ancient DNA is quite a challenge. Although more and more research centers undertake the task, there is still little knowledge on this matter. First step of analysis is finding of certified aurochs bones or teeth and isolation of DNA. That was the most important step, followed by cloning of aurochs DNA in bacterial system for further analysis encompassing DNA sequencing and comparison with the existing bovine database in search for any similarities and specific genes. Some of the results have been already published. Studies of aurochs help also understanding history of species, its relation to other species and perhaps will help preventing extinction of other animals.
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The genetics of the XXI century

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In recent years, two developments revolutionized the molecular genetics. The first one is an enormously improved technique of the DNA sequencing. It is now possible to obtain in couple of hours and on the low cost, the full sequence of the human genome. The second one is the invention of the CRISPR-Cas technique, which allows the precise manipulations of genomes of living organisms. This technique was already successfully applied to “repair” human genes responsible for hereditary diseases. To some astonishment, applications of genetic engineering to humans does not bring protests of general public, while similar manipulations pf plants genomes are considered an dangerous. In Poland and in some other countries, parliaments introduced an extremely restrictive laws, which in practice makes cultivation of GM plants outside laboratories impossible. This is caused by the ignorance of the general public and by opposition of catholic church which considers genetic modifications of all organisms as contradictory with Gods will. The most common modification of plants is an introduction into their genomes of the single gene from the bacterium Bacillus thuringensis, what make the plant resistant to harmful insects. According to WHO, neither the manipulation mentioned above, nor manipulations of the other kind, do not pose any danger to humans.
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An overview of the methods used by the authors of Chapter 5 from a monograph on analysis of the Globular Amphorae culture remains from the Koszyce site 3 (M. Kuś, A. Ossowski 2013) suggests a series of methodological objections. DNA analysis of the same material performed in accordance with generally accepted rigors of ancient DNA research raises doubts about authenticity of the published sequences and their interpretation.
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W poszukiwaniu Piastów

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The origin of the Piast dynasty is a matter of lively discussions and disputes. At least a few controversial hypotheses exist, but their credibility is difficult to assess due to the scarcity of written as well as material sources, especially from the time of Polish state formation. Life sciences, however, can support history and archeology. Application of genetic tests, used earlier mainly in forensic laboratories, enabled identification of the remains of King Richard III, the Romanov dynasty members and Nicolaus Copernicus. Contemporary DNA studies, based on next generation DNA sequencing, outreach the narrow area of known markers such as mitochondrial DNA (mtDNA) and selected regions of Y chromosome. Although ancient DNA (aDNA), extracted from remains, is usually highly degraded and contaminated with genetic material of microorganisms, there are methods which allow for the analysis of such material and retrieval of information about origin, kinship and some phenotypic features of an individual. Genetic studies of the Piast dynasty, a subject of our research project, have to deal with numerous difficulties. In or der to gain access to bone samples, we need to meet a number of formal requirements. Moreover, despite the existence of available abundant documentation on the Piast burials, the actual situation is not always consistent with the written sources. Our first experiences show how difficult it is to localize the remains, identify them and extract DNA of sufficient quality.
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