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Influence of pressure changes during embryo transfer on blastocyst viability.

100%

Mrugacz G., Grygoruk C., Gajda B., Grad I., Grusza M., Sieczynski P.

Progress in Health Sciences

2013

vol. 3

issue 1

null-89

Purpose: The embryo transfer into the uterus by a transcervical catheter is the final stage of in-vitro fertilization procedure. So far, a little attention has been placed on the impact of embryo transfer procedure on embryo viability. The study was design to measure the morphological changes and apoptosis rate in embryos exposed to embryo transfer in in-vitro conditions. Methods: Morphologically normal rabbit blastocysts were divided randomly into control (48) and experimental (48) groups. The experimental group blastocysts were exposed to embryo transfer in in-vitro conditions. Morphological changes in response to embryo transfer were assessed 5 and 60 minutes after ET. The apoptosis rate was measured one hour after embryo transfer. Results: Morphological changes in response to embryo transfer were more prevalent in the experimental group; 14 shrunken and one collapsed blastocysts in experimental group and only two shrunken blastocysts in the control group. The mean DNA fragmented nucleus index in the experimental group was 37.7 % and was significantly higher than in the control group, 8.1 %. Conclusions: Embryo transfer can trigger both morphological and apoptotic changes in rabbit blastocysts. The local pressure fluctuations during embryo transfer could be a major factor responsible for the above-mentioned changes.

Protective role of mucin coat.

88%

Grygoruk C., Mrugacz G., Modliński, J.A, Gajda B., Grad I., Grusza M., Sieczynski P.

Progress in Health Sciences

2013

vol. 3

issue 1

83-88

Purpose: The present study was designed to examine the protective property of the mucin coat against pressure fluctuation. Materials and Methods: Thirty non-hatched rabbit blastocysts containing mucin coat and thirty mouse blastocysts were exposed to pressure fluctuations in in-vitro conditions. Morphological response of the blastocysts was assessed 5 and 60 minutes after exposition to pressure fluctuation. Results: Out of thirty mouse blastocysts there were 4 collapsed in 5th minute and 8 collapsed in the 60th minute. There were no collapsed rabbit blastocysts in 5th minute and only one in 60th minute. The difference between groups was statistically significant; p<0.05. Conclusions: Mucin coat has a unique property of protecting embryo from a deleterious effect of the rapid pressure fluctuations.

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2 Progress in Health Sciences

2 Gajda B.

2 Grad I.

2 Grusza M.

2 Grygoruk C.

2 Mrugacz G.

2 Sieczynski P.

1 Modliński, J.A

2 2013

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Influence of pressure changes during embryo transfer on blastocyst viability.

Protective role of mucin coat.