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Enzymatic activity and biotypes of Candida fungi isolated from the surfaces of mobile phones and hands

100%
Kordecka A., Krajewska-Kułak E., Elżbieta, Łukaszuk C., Kraszyński M., Kraszyńska B.
Progress in Health Sciences
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2017
|
vol. 7(1)
18-30
EN
Introduction: The secretion of hydrolytic enzymes is a factor facilitating pathogenic fungi invasion into the tissues. Purpose: To assess hydrolytic activity and biotypes of Candida strains isolated from samples collected from the surfaces of mobile phones and the hands of their owners. Materials and methods: The study included 175 mobile telephones and hands. The API ZYM test was used to assess enzymatic activity; biotyping was performed according to Williamson’s classification. Results: Among the strains isolated from hand surfaces, the highest activity was shown for C. albicans (acid phosphatase, esterase), C. glabrata (leucine arylamidase, acid phosphatase, esterase), and C. krusei (acid phosphatase). Of the strains isolated from phone surfaces, the highest activity was shown for C. albicans (leucine arylamidase, acid phosphatase), C. glabrata (esterase, leucine arylamidase, esterase lipase), and C. krusei (acid phosphatase). Biotypes G, B and F were dominant for all types of fungi, both for strains isolated from phones and hand surfaces. Additionally, biotype A was dominant for C. krusei. Conclusions: C. albicans, C. glabrata, and C. krusei showed activity for all hydrolytic enzymes. The strongest correlation between the hydrolytic activity of fungi isolated from hand and phone surfaces was shown for C. albicans.
2
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Drug susceptibility and enzymatic activity of Candida isolated from mobile phone and hand surfaces

88%
Kordecka A., Krajewska-Kułak E., Łukaszuk C., Kraszyński M., Kraszyńska B.
Progress in Health Sciences
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2017
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vol. 7(2)
7-18
EN
Introduction: Evaluation of susceptibility of isolated fungi as well as the type and activity of enzymes they release allows to determine their pathogenicity. Purpose; To assess potential correlations between drug susceptibility and enzymatic activity of strains isolated from mobile phone and hand surfaces Materials and methods: The mycological evaluation included 175 mobile phones and 175 hands of the phone owners. Drug susceptibility was assessed using the FUNGITEST; enzymatic activity was evaluated using the API ZYM test. Results: We found significant correlations between an increased resistance to 5-fluorocytosine, ketoconazole, fluconazole and higher activity of six selected enzymes for Candida glabrata strains isolated from hand surfaces. We found also significant correlations between an increased resistance to 5-fluorocytosine, ketoconazole, miconazole, itraconazole and higher activity of six selected enzymes for Candida albicans strains isolated from hand surfaces. We found significant correlations between an increased resistance to 5fluorocytosine, ketoconazole, itraconazole, fluconazole and higher activity of six selected enzymes for Candida krusei strains isolated from hand surfaces as well as an increased resistance to 5-fluorocytosine, ketoconazole, itraconazole, fluconazole and higher activity of five selected enzymes for strains isolated from phone surfaces. Conclusions: We found varying correlations between enzymatic activity and drug resistance depending on the site of isolation and the species/genus of fungi. The drugs to which the evaluated strains showed resistance were the same for hand and mobile phone isolates.
3
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The prevalence and risk factors of musculoskeletal disorders in the hands of fishermen working as oyster shuckers

75%
Park J. S., Yoo J.-I., Na J.-B., Song H. S.
International Journal of Occupational Medicine and Environmental Health
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2021
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vol. 34
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issue 5
603-615
EN
ObjectivesThis study aimed to investigate the prevalence of musculoskeletal disorders in the hands and wrists of fishermen working as oyster shuckers, and to identify the risk factors associated with oyster shucking. Material and Methods A total of 590 fishery workers including those working as oyster shuckers in 2015–2017 were included in the study analysis. Hand osteoarthritis (OA), carpal tunnel syndrome (CTS), and ulnar impaction syndrome were diagnosed using X-ray, magnetic resonance imaging, and physical examinations according to each diagnostic criterion. The Rapid Entire Body Assessment was used as a measurement tool for analyzing the occupation-related burden factors.ResultsThe prevalence of hand OA, CTS, and ulnar impaction syndrome was higher in oyster shuckers than in aquaculture/offshore fishery workers. The prevalence of upper limb disease was higher in oyster shuckers than in other fishery workers, even in the prevalence of exposure to occupational burden factors. In oyster shuckers, the incidence of hand and wrist OA was 84%, that of CTS was 72%, and that of ulnar impaction syndrome was 41%. In addition, oyster shuckers were more likely to be exposed to occupational burden factors than were other types of fishermen. Conclusions The prevalence of hand OA, CTS, and ulnar impaction syndrome was higher in oyster shuckers. Therefore, proper stretching or exercises for rest and musculoskeletal relaxation, along with health-care education and promotion, will be needed for the fishermen’s repeated posture and impact work.
4
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Zmartwychwstały Pan Jezus – Radość Ewangelii (J 20, 20b)

38%
Witczyk H.
Łódzkie Studia Teologiczne
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2016
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vol. 25
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issue 3
5-24
EN
The author analyses exegetically J 20, 19–20 where the disciples were filled with joy when they saw Risen Jesus. Starting from the analysis of the literary context and the motives related to the disciples’ joy, the author stresses church-centered role of the Risen Lord (kyrios). He points out a number of references to the appearances of Risen Christ in Luke 24, which seem to have many points in common with the Johannine tradition. He particularly focuses on the very moment of appearance when Jesus in a symbolic gesture shows his hands and side to the disciples. The hands and side are the symbols of tender love of the Good Shepherd and inexhaustible source of the Holy Spirit. Moreover, he presents the contrast between the experience of fear, confusion and scattering (before the Resurrection) and the experience of joy, confidence and unity which was rediscovered around the Risen Jesus.
IT
L’articolo presenta un’analisi esegetica di Gv 20, 19–20, dove gli apostoli si rallegrarono al vedere Gesù risorto. Si esamina il contesto letterario, i motivi inerenti alla gioia dei discepoli e il ruolo ecclesiocentrico del Signore Risorto (kyrios). Non mancano riferimenti al racconto delle apparizioni in Lc 24, che sembra molto vicino alla tradizione giovannea. Un’attenzione particolare viene data al momento dell’apparizione, quando Gesù, eseguendo un gesto simbolico, mostra ai discepoli le mani e il costato, entrambi i simboli della amorevole cura del Buon Pastore e dell’inesauribile fonte dello Spirito Santo. Inoltre, viene messo in risalto il contrasto tra lo stato di paura, smarrimento e dispersione (prima dell’apparizione del Risorto) e lo stato di gioia, fiducia e ritrovata unità attorno a Gesù Risorto.
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