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Assessment of prevalence of HPV DNA in colorectal adenocarcinoma in patients from the Podlasie region

100%
Miąsko A., Chomczyk M., Pancewicz–Wojtkiewicz, J, Roszkowski A., Kędra B., Borzym - Kluczyk M., Szynaka B., Chyczewski L.
Progress in Health Sciences
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2012
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vol. 2
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issue 2
84-89
EN
Purpose: The aim of the study was to assess the prevalence of human papillomavirus (HPV) DNA in colorectal adenocarcinoma in patients from the Podlasie region undergoing surgery for the tumor. Materials and methods: We examined 40 solid colorectal tumors taken during surgical treatment at the 2nd Department of General and Gastrointestinal Surgery, Medical University of Białystok. HPV was detected by means of polymerase chain reaction (PCR) and in situ hybridization (ISH). The tests were performed on formalin-fixed, paraffin-embedded tumor tissue. Two pairs of primers were used for the detection of HPV DNA by PCR. Pair pU-1M/pU-2R enables detection and identification of high-risk HPV (HPV 16, 18, 31, 33, 35, 52b, 58), while pair pU-31B/pU-2R enables detection and identification of low-risk HPV (HPV 6, 11). The ISH was performed with the use of biotin-labelled dsDNA probes, using Wide Spectrum HPV DNA Probe Cocktail Biotinylated kit, DAKO Cyto-mation. Results: HPV DNA was found in 21 (52.5%) of the examined colorectal tumors. The PCR revealed the presence of viral DNA in 19 (47.5%) tumors. The ISH revealed the presence of HPV DNA in 16 (40%) of the examined tumors. Conclusion: The findings of this study correlate with similar results conducted by other research groups. However, this is the first study of colorectal tumor samples taken from patients of the Podlasie region. Therefore, the association between environmental factors, HPV infection, and tumor stage should also be verified in a larger study population. Further studies confirming the presence of HPV DNA in colorectal tumor tissue in populations from different regions of Poland are needed.
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Acute SARS-CoV-2 infection and seropositivity among healthcare workers and medical students in summer 2020, Hungary

75%
Kosztin A., Merkely B., Szabó A. J., Blaha B., Varga P., Vásárhelyi B., Vokó Z.
International Journal of Occupational Medicine and Environmental Health
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2021
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vol. 35
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issue 2
209-216
EN
ObjectivesThe aim was to compare the prevalence of acute infection and seropositivity of SARS-CoV-2 among healthcare workers (HCWs) and medical students.Material and MethodsA high-volume, single-center analysis was conducted in the period of July 1‒August 1, 2020, at the Semmelweis University. Naso- and oropharyngeal samples were collected for polymerase chain reaction (PCR), and blood samples for anti-SARS-CoV-2 IgG. A questionnaire was also administered about the infection symptoms and the obtained results were assessed by profession and site of care delivery.ResultsFrom the total cohort (N = 7948), 4478 (56%) and 3470 (44%) were health professionals and medical students, respectively. They were mainly female (67%), and the mean age of HCWs and students was 40 and 25 years, respectively. By profession, physicians (1.5%) and other HCWs (1.8%) showed a comparable SARS-CoV-2 exposure. International students had the highest (2.1%), whereas Hungarian students had the lowest (0.6%) prevalence of seropositivity. The highest prevalence was detected among the staff of COVID-19 wards (12.1%). By PCR, medical students showed the lowest occurrence of active infection with a prevalence of 0.17%, while physicians and other HCWs had a higher prevalence (1.46% and 1.71%, respectively). By site of care delivery, positive test results were the most frequent at COVID-19 wards (3.8%).ConclusionsPhysicians and other HCWs showed comparable SARS-CoV-2 seropositivity prevalence, approximately twice as high as in the general population of Budapest. Hungarian students had lower prevalence of seropositivity than this reference. High prevalence among international students suggests that they had imported the infection. The very high prevalence of documented exposure among staff members at COVID-19 wards urges for improving the safety measures.
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