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1

Serum and urinary β-glucuronidase in acute alcohol intoxication: a pilot study

100%
Waszkiewicz N., Zalewska-Szajda B., Buras A., Szulc A., Zwierz K., Ładny J., Szajda S.
Progress in Health Sciences
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2012
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vol. 2
|
issue 2
52-56
EN
Introduction: Beta-glucuronidase (GLU) is a member of the lysosomal glycosidase family that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of glycosaminoglycans. Increased activities of GLU have been earlier reported in the serum of alcohol-dependent patients after a chronic heavy drinking period but not after acute intoxication (called binge drinking). The accelerating binge drinking pheno-menon is an alarming public health issue that requires better prevention. Purpose: To determine the activity serum and urinary GLU, after an acute, single, and a large dose of alcohol intoxication. Materials and methods: The serum and urine of eight healthy binge drinkers were collected before binge drinking, and 2 and five days after the drinking session. The activity of GLU was determined by the colorimetric method. Results: There was a tendency to decrease in the serum GLU activity two days after acute alcohol intoxication (binge drinking), which was followed by the significant increase in the GLU activity five days after drinking. The urinary activity of GLU was not changed after intoxication. Conclusion: Alcohol-induced imbalance in the serum GLU activity might be associated with alcohol-induced liver hypoxia and subsequent reperfusion, and can be detected even five days after the drinking session.
2

Serum and urinary carnitine in children with cystic fibrosis

84%
Kępka A., Minarowska A., Minarowski Ł., Ł, Waszkiewicz N., Chojnowska S., Trochimowicz L., Zwierz K., Chyczewska E., Szajda, Sł. D
Progress in Health Sciences
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2013
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vol. 3
|
issue 1
13-18
EN
Purpose: Cystic fibrosis (CF) is inherited, congenital disease of multi-organ expression. Carnitine play a role as a lipid acid transporter to mitochondrium for beta-oxydation. Acylation of carnitine is inevitable for detoxication processes in cells. Low lean body mass In CF patients can lead to decreased levels. The aim of the study was the evaluation of free carnitine, acylcarnitine and acylcarnitine/free carnitine ratio in serum and urine of children with cystic fibrosis. Material and methods: The study was conducted in a group of 15 CF children (4 F, 11 M), aged 12.6 ±5.4 years. The serum for a control group was collected from 32 healthy children. Urine samples for control group was collected from 62 health children. Free carnitine and total carnitine was assessed using spectrophotomeric method in which acyl group is transferred from acetyl-CoA to carnitine by carnitine acetyltransferase (CAT). Acyl carnitine concentration and acylcarnitine/free carnitine ratio was counted using Schmidt- Sommerfeld and Seccombe equation. Results: In 12 CF patients (80%) free carnitine and total carnitine was below lower limit of normal (p<0.001). In 9 patients (60%) free carnitine level was ≤ 20 µmol/L, which can be clinically diagnosed. Acylcarnitine levels were also statistically lower in CF group (p<0.01). Acylcarinitie/free carnitine ratio did not differ between the groups (p=0.05). Urine excretion of free carnitine, total carnitine and acylcarnitine was lower in CF group (p<0.001). Conclusions: In CF pediatric patients statistically significant lower levels of free carnitine, total carnitine and acylcarnitine were observed in comparison to controls. Low urine excretion of free carnitine, total carnitine and acylcarnitine was lower in CF group. No correlation between serum and urine levels of free carnitine, total carnitine and acylcarnitine were observed.
3

Lyme disease: etiology, pathogenesis, clinical courses, diagnostics and treatment

84%
Wasiluk A., Zalewska-Szajda B., Waszkiewicz N., Kępka A., Szajda D., Wojewódzka-Żeleźniakowicz M., Ładny J., Pancewicz S., Zwierz Z., Zwierz K.
Progress in Health Sciences
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2011
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vol. 1
|
issue 2
179-186
EN
Lyme disease (LD) is caused by Borrelia burgdorferi, transferred by infected ticks Ixodus ricinus. LD occurs endemically in Europe, America and Northern regions of Asia. In Poland, LD is the most frequent tick borne disease, which causes serious epidemiological problems. The main health hazard of LD occurs on the forested areas of Podlasie, Maritime province, the West Poland lake district, and Carpathians. The highest incidence 114.0 per 100 000 was registered in Podlaskie province. The infectious cycle of Borrelia burgdorferi includes: nymph moulds to adult, eggs laid by female, eggs hatch to larva, larva feeds on first host, fully fed larva drops to ground, larva moulds to nymph, nymph attaches to and feeds on the second host. LD is a chronic disease attacking many organs, including the skin, heart, brain and joints. LD is divided into three stages based on clinical symptoms: I - limited infection (Erythema migrans and Borrelia lymphoma), II - disseminated infection (numerous erythema migrans, early neuro-borreliosis, joint inflammation, Lyme carditis), III - late borreliosis (chronic atrophic limbs infla-mmation, late neuroborreliosis, chronic joint infla-mmation). At present, the best diagnostic method for LD is a labor- and time consuming two-stage serological method. First-line antibiotics in the treatment of Lyme disease are doxycycline, amoxicillin, cefuroxime axetil, ceftriaxone, cefo-taxime, and penicillin G. The best method of avoiding Borrelia burgdorferi infection is to avoid biting from ticks carrying spirochetes. Early removal of ticks, protects against spirochaetal infection.
4

Serum beta-glucuronidase in colon cancer patients dependent on alcohol and nicotine: preliminary report

84%
Waszkiewicz N., Zalewska-Szajda B., Repka B., Szulc A., Kępka A., Chojnowska S., Dadan J., Ładny J., Zwierz K., Szajda S.
Progress in Health Sciences
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2012
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vol. 2
|
issue 2
64-69
EN
Introduction: Beta-glucuronidase (GLU) is a lysosomal exoglycosidase involved in the catabolism of glycoconjugates. Excessive GLU activity may be a primary factor in the etiology of colon cancer. The stimulation of glycosidases and other degradative enzyme activity has been noted in cancers as well as in alcohol and nicotine addiction. Purpose: To compare the serum GLU activity between alcohol- and nicotine-dependent colon cancer patients and colon cancer patients without a history of alcohol- and nicotine-dependence. Materials and methods: Material was the serum of 22 colon cancer patients, 11 of whom met alcohol and nicotine dependence criteria. The activity of serum GLU (pKat/ml) was determined by the colorimetric method. Carcinoembryonic antigen (CEA) concentration (ng/ml) in the serum was determined by the immunoenzymatic method. Comparisons between groups were made using the Mann-Whitney “U” test. Spearman’s rank correlation coefficient was used to measure the association between two variables. Results: The activity of serum GLU was significantly higher in colon cancer patients with a history of alcohol and nicotine dependence, than in the colon cancer patients without a history of drinking and smoking (p=0.003). There was no significant difference in the CEA concentration between colon cancer patients with and without a history of drinking and smoking. Conclusion: Alcohol and nicotine addiction may increase the serum GLU activity in all cancer patients, as already seen in colon cancer patients. This may potentially be important for the degradation of pericancerous matrix, tumor growth, invasion and metastasis
5

Etiopathogenesis of nasal polyps

68%
Chojnowska S., Kępka A., Waszkiewicz N., Kołodziejczyk Z., Konarzewska-Duchnowska E., Ościłowicz K., Cabaj-Wiater I., Sowizdraniuk J., Dzięcioł J., Ładny J., Zwierz K., Szajda S.
Progress in Health Sciences
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2013
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vol. 3
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issue 2
151-159
EN
Nasal polyps are soft, pink or pearl white outgrowths usually connected with nasal mucosa by flaccid crus. A histological image of nasal polyps indicates significant differences in particular cases. Because of the variety of nasal polyps, the choice of proper laryngological treatment is very difficult. Difficulties in treatment are due to the lack of consensus among physicians regarding the etiopathogenesis of nasal polyps. Probably etiopathogenesis of nasal polyps is not homogeneous and polyps’ formation is influenced by many coexisting mechanisms.
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