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Purpose: Enterococcus faecium strains have been reported worldwide as etiologic factors of many nosocomial infections, which are difficult to manage because of the constantly increasing resistance of these microorganisms to antibiotics and the ability to form biofilm. The aim of this study was to analyze the ability to produce a biofilm in E. faecium strains, depending on the patient’s clinical material. Materials and methods: Sixty-six E. faecium strains were investigated. Identification and susceptibility testing were conducted by the VITEK2 system. The ability to form biofilm was assessed by phenotypic methods. The presence of selected virulence genes was established by PCR followed by gel electrophoresis and sequencing. Results: Among the tested E. faecium isolates, 72.7% were biofilm-positive (BIO+) and 27.3% biofilm-negative (BIO-). Strains were collected mostly from rectal swabs (30.4%) and blood (18.3%). BIO+ strains from infections constituted 31.8% (52.4% isolated from blood) and from colonization 40.9% (48.2% from rectal swabs). 91.7% of the Blood Group strains and 68.5% of the Other Group strains produced biofilm. Strains from the Colonization Group produced biofilm in a proportion similar to the Infection Group (about 75%). There were no statistically significant differences in virulence and resistance, except for vancomycin (more resistant BIO+ Other than the BIO+ Blood Group, and more resistant BIO+ Colonization than BIO+ Infection Group) and teicoplanin (more resistant BIO+ Colonization than the BIO+ Infection Group). Conclusion: The majority of E. faecium isolates carries high levels of resistance to many antimicrobials, is well equipped with virulence genes, and possesses the ability to form biofilm.
EN
Introduction: Pseudomonas aeruginosa rods are increasingly causing serious infections in hospitalized patients. Particularly worrying is the increase of resistance to carbapenems antibiotics. Purpose: To evaluate susceptibility and the occurrence of genes (blaIMP and blaVIM) encoding resistance to carbapenems among Pseudomonas aeruginosa strains. Materials and methods: Studies were conducted for 50 strains of Pseudomonas aeruginosa (25 susceptible and 25 resistant to imipenem). Susceptibility to antibiotics was tested using the diffusion method and discs with antibiotics and / or strips with gradient concentrations of antibiotics. In the second phase we tested the ability of MBL production by all strains using the CD and DDST technique described in the literature. The next stage of the study was to evaluate the prevalence of carbapenems resistance genes. These studies were performed by PCR technique. Results: The studies found in both groups of Pseudomonas aeruginosa rods similar percentage of strains resistant to aminoglycoside antibiotics (from 72% to 88%) and ciprofloxacin (84%). There was no presence of the genes in any of the tested groups of Pseudomonas aeruginosa. Conclusion: Pseudomonas aeruginosa strains resistant to imipenem no posses blaIMP and blaVIM genes therefore their resistance was conditioned by the presence of other mechanisms. Antibiotics with high activity against Pseudomonas aeruginosa strains resistant to imipenem were polymyxin B (100% susceptible strains) and colistin (96% susceptible strains).
EN
The increasing prevalence of acquired carbapenemases in Gram – negative bacteria is one of the biggest problems in the prevention and therapy of infectious diseases. NDM (New Delhi Metallo--Lactamase) is a recently discovered enzyme which has the ability to hydrolyze all -lactam antibiotics, except aztreonam. Making that scenario more worrisome is the fact that mobile fragments of DNA carrying blaNDM genes, also keeps a number of other genes encoding antibiotic resistance. NDM enzymes are currently present in different species of bacteria all over the world. NDM-producing bacteria are resistant to virtually all available antimicrobial agents except tigecycline, colistine and fosfomycine
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