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1

Daniel Guérin o Róży Luksemburg i spontaniczności rewolucyjnej.

100%
Kochan E.
Nowa Krytyka
|
2016
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issue 36
93-111
EN
The article contains a critical presentation of Daniel Guérin’s work: Rosa Luxemburg and revolutionary spontaneity publushed in 1971. Daniel Guérin accuses Rosa Lusemburg of theoretical inconsequence and disorder. These accusations are presented in the light of critical approach towards Guérin’s narrowminded anarchism and its line.
2

Róża Luksemburg i bunt mas

100%
Kochan E.
Nowa Krytyka
|
2015
|
issue 34
67-81
EN
The subject of this article is the concept of the masses in the social theory of Rosa Luxemburg. In this approach the masses are a big collective and a subject of social transformation, and the revolution. These masses also possess a specific wisdom (social and political instinct) and they act spontaneously. In this article I also present the positions of Luxemburg’s critics and my own opinion about the meaning of her theory. It is my opinion that Rosa Luxemburg’s concept should be viewed as a „transistional moment”, and as a necessary requirement for a marxist theory of ideology and social consciousness.
3
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Panax quinquefolium hairy root extracts and their effect in connections with antibiotics against pathogenic bacteria – preliminary study

100%
Kochan E.
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
|
2018
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vol. 73
|
issue 1
EN
 The aim of the present study was to determine the level of ginsenosides in extracts from hairy root A, B, G clones of Panax quinquefolium and their action with antibiotics against clinical bacterial isolates. The content of ginsenosides (the key biologically active compounds) were determined in tested extracts using HPLC. The activity of extracts with antibiotics was established by micro-dilution broth method. Total triterpene saponin content was 14.68, 14.32 and 10.07 mgg-1 d.w. for root culture clones B, A and G, respectively. Our research indicates that the addition of extracts mainly from B and G clone hairy root cultures to antibiotics allow to reduce the ampicillin and tetracycline effective concentration respectively against Enterococcus faecalis and both Escherichia coli and Acintobacter baumannii.
4
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Influence of methyl jasmonate on ginsenoside biosynthesis in suspension cultures of Panax quinquefolium L.

39%
Kochan E., Caban S., Szymańska G., Szymczyk P., Lipert A., Kwiatkowski P., Sienkiewicz M.
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
|
2017
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vol. 72
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issue 1
EN
Panax quinquefolium L., belonging to the Araliaceae family, along with P. ginseng is one of the well-known species of ginseng. Multidirectional pharmacological action of this plant is attributed to triterpene saponins called ginsenosides. Pharmacopoeial raw material are roots obtained from the field crops which are time-consuming and require expensive agrotechnical procedures. Therefore, the new sources of ginseng biomass are sought such as in vitro suspension cultures. P. quinquefolium L. cell cultures, treated with the elicitation of methyl jasmonate (MJ) in concentration 50 and 250 μmol L-1, synthesize more ginsenosides than control cultures. The highest increase (2.2-fold) of all examined compounds was noted using 250 μmol L-1 MJ. In this condition, the predominantly quantitative metabolite was Rb1 ginsenoside belonging to protopanaxadiol derivatives.
5
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Ginsenoside content in suspension cultures of Panax quinquefolium L. cultivated in shake flasksand stirred-tank bioreactor

39%
Kochan E., Caban S., Szymańska G., Szymczyk P., Lipert A., Kwiatkowski P., Sienkiewicz M.
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
|
2017
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vol. 72
|
issue 1
EN
Plant suspension cultures are described as a source for the acquisition of medicinal secondary metabolites which in the future may become an alternative to traditional raw materials. This study demonstrates that the cell cultures of one of the ginseng species – Panax quinquefolium L. synthesize ginsenosides, which are triterpene saponins having a multidirectional pharmacological effects. Tested suspension cultures were run on a small scale in the shake flasksand in scale up of the process in a 10-liter stirred tank. In the shake flasks,the highest biomass yield (2.28 gl-1 for dry and 33.99 gl-1 for fresh weight) was reached on day 30 of culture, and the highest content of saponins (2.66 mg g -1 dw) was determined on day 28 of culture. In the bioreactor, nearly 2.67 and 3-fold increase of respectively dry and fresh biomass was recorded in relation to the inoculum. Large-scale cultures synthesized protopanaxatriol derivatives such as Rg1 and Re ginsenosides, however, no saponins belonging to the protopanaxadiol derivatives were reported.
6
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Physical activity of future health care professionals: adherence to current recommendations

39%
Lipert A., Matusiak-Wieczorek E., Kochan E., Szymczyk P., Wrzesińska M., Jegier A.
Medycyna Pracy
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2020
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vol. 71
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issue 5
539-549
EN
BackgroundWhen assessing physical activity (PA), particular attention should be paid to medical university students who are taught to be health care professionals (HCPs) responsible for maintaining health in humans. However, different studies have shown that HCPs exhibit the same unhealthy behaviors as the general population. This study analyzed PA among medical university students of different faculties and their adherence to current PA recommendations.Material and MethodsData from 216 medical university students of physiotherapy, dietetics and pharmacy, including males (N = 44) and females (N = 172), the mean age of 22.3±1.8 years, were collected. The International Physical Activity Questionnaire in its long form (IPAQ-LF) was used to assess and classify PA behaviors. The results were analyzed in accordance with World Health Organization recommendations regarding PA.ResultsOver 60% of all the students were classified as active during all-day activity. However, while analyzing PA in different domains, the same shares of all the students were still insufficiently active during leisure time, and so they did not meet the recommendation of >75 min/week of vigorous PA, >150 min/week of moderate PA or an equivalent combination. All the students self-reported PA mainly in the work and transport domains. Generally, physiotherapy students were the most active and performed PA with higher intensity.ConclusionsThis study revealed a low level of leisure time PA among the students, and no habit of regular PA. Some changes in medical education should be suggested to include physical education as a long-term subject in medical school curricula. Future research is needed to investigate the exercise barriers that students perceive, which can guide future interventions aimed at improving their PA, and thereby impact on the quality of health care which they will provide.
7
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Antibiotic resistance among Escherichia coli urinary isolates and their susceptibility to clove essential oil

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Dąbrowski M., Sienkiewicz M., Zielińska-Bliźniewska H., Dąbrowska M., Seredyńska M., Kochan E.
Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia
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2016
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vol. 71
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issue 2
EN
Escherichia coli is a Gram-negative, facultatively anaerobic, rod-shaped, coliform bacterium, which is a primary cause of urinary tract infections. Resistance to antibiotics has become a particular problem in recent decades. Consequently, there is an unmet need for new therapeutic options. It has been observed that essential oils have bactericidal effects. The antimicrobial susceptibility testing for Escherichia coli isolates obtained from urine of patients with urinary tract infections was determined via disk diffusion method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST, 2015). Essential oil from clove – Syzygium aromaticum (L.) Merill et L.M. (Myrtaceae) was analyzed by GC-FID-MS. Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) were detected by using the micro-dilution broth method. Escherichia coli clinical isolates are characterized by high resistance to ampicillin, amoxicillin with clavulanic acid, norfloxacin, trimethoprim/sulfamethoxazole, tetracycline, tobramycin and ticarcillin. Clove oil possesses strong inhibiting and killing properties against E. coli isolates, among them the ones resistant to recommended antibiotics. The results of this study highlight the need for testing the efficacy of new agents to inactivate bacteria in clinical settings.
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