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Usefulness of computed tomography performed after forensic autopsy in the diagnosis of the battered child syndrome: a case report

100%
Skowronek R., Ptak D., Pilch-Kowalczyk J.
Problems of Forensic Sciences
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2022
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vol. 132
EN
Diagnostic imaging methods routinely used in clinical practice (mainly multi-slice computed tomography, CT) are increasingly entering forensic practice, also in Poland. Typically these exams are performed prior to an appropriate external examination and autopsy. The aim of the study is to present a case in which computed tomography examination performed only after forensic medical autopsy turned out to be useful in the diagnosis of an abused child syndrome. The CT examination showed, inter alia, nighstick injury/fracture of the ulna in its distal part, and deformation of the base of the phalanx of the distal finger of the fourth left hand, corresponding to a fracture/crush. Post-mortem imaging may be useful in the case of a suspected abused child syndrome.
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Expression of cytochrome P450 2C and 3A in female rat liver after long-term administration of gonadoliberin analogs

76%
Skowronek R., Czekaj P., Suszka-Świtek A., Czech E., Wiaderkiewicz A., Plewka D., Bryzek A.
International Journal of Occupational Medicine and Environmental Health
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2015
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vol. 29
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issue 2
293-314
EN
Objectives Gonadoliberin (GnRH) analogs may be expected to indirectly modify growth hormone (GH) total concentration and its 24-h secretion profile. As a consequence, changes in the levels of GH may modify the mechanism of sexdependent cytochromes P450 (CYP450) synthesis, including the expression of transcriptional factors. The aim of the study has been to evaluate the effect of long-term administration of a low dose of GnRH analogs on hepatic expression of CYP2C and CYP3A isoforms, and the transcription factors: pregnane X receptor (PXR), hepatocyte nuclear factor 4α (HNF4α), HNF6 and signal transducers and activators of transcription 5b (STAT5b). Material and Methods The study was carried out on adult female Sprague-Dawley rats during a 3-month treatment with dalarelin (GnRH agonist) and cetrorelix (GnRH antagonist), at a daily intraperitoneal injection (i.p.) dose of 6 μg/kg body weight/day, and 1, 2, and 4 weeks after treatment discontinuation. The concentrations of ovarian hormones and GH in the blood serum were determined by radioimmunoassay and enzyme-linked immunosorbent assay (ELISA) method, respectively. Then, the expression of hepatic CYP450s (reverse transcription polymerase chain reaction – RT-PCR, Western blot and immunohistochemistry) and transcription factors (RT-PCR) was evaluated. Results We have found that cetrorelix induces changes in the circadian pattern of GH secretion and enhances GH blood concentrations. These changes may cause increased expression of both, female-specific CYP450s (especially CYP3A9), and HNF4α/HNF6 transcription factors. Decrease in GH blood concentrations, resulting from the effect of dalarelin, may promote inhibition of female-specific CYP2C12 and CYP3A9 isoforms as well as STAT5b transcription factor. Slight changes in sex-independent CYP3A1 protein expression caused by GnRH analogs were also observed. Conclusions In adult female rats, HNF4α/HNF6 and STAT5b seem to be crucial for the regulation of GnRH antagonist/GH- and GnRH agonist/GH-dependent pattern of CYP450 expression, respectively.
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