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Heavy metal and mould contamination of herbal medicinal products – an overview

100%
Ałtyn I., Twarużek M.
Annales Universitatis Paedagogicae Cracoviensis Studia Naturae
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2018
EN
Rośliny zielarskie są powszechnie wykorzystywane jako surowce w przemyśle farmaceutycznym. Od czasów starożytnych ziół używa się w leczeniu i zapobieganiu chorób. Jednakże w dzisiejszych czasach mogą one nie spełniać wymagań dotyczących jakości, bezpieczeństwa i skuteczności. Większość produktów ziołowych nie jest testowana, a ich efekty działania są słabo monitorowane. Konsekwencją tego jest niewystarczająca wiedza na temat przebiegu ich działania, skutków ubocznych, przeciwwskazań oraz interakcji z występującymi na rynku produktami farmaceutycznymi oraz żywnością. Przyczyną negatywnego wpływu surowców ziołowych na organizm może być ich zanieczyszczenie przez różne grzyby pleśniowe, powstające podczas zbioru, przetwarzania, przechowywania, a także dystrybucji. Zanieczyszczenie surowców zielarskich może być również spowodowane przez różnorodne metale ciężkie, które występują w wielu aspektach współczesnego życia. Celem niniejszej pracy jest przegląd informacji na temat stanu mykologicznego i chemicznego roślin leczniczych, a także wskazanie kilku ważnych wyzwań związanych z efektywnym monitorowaniem ich bezpieczeństwa.
2
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Mycotoxin – induced apoptosis in swine kidney epithelial cells

81%
Chrustek A., Twarużek M., Zastempowska E., Grajewski J.
Annales Universitatis Paedagogicae Cracoviensis Studia Naturae
|
2016
EN
Apoptosis, as the programmed cell death, plays a significant role in proper functioning of an organism, both in the postnatal period and during embryogenesis. Disturbances in this process can lead to the occurrence of several dysfunctions, e.g. cancer, stroke, Alzheimer’s disease and others. Apoptosis can be triggered by factors such as oxidative stress, free radicals, UV radiation and cytotoxic drugs, but also mycotoxins, e.g. aflatoxins, ochratoxin A and trichothecenes. These toxins are produced primarily by fungi of Aspergillus, Penicillium, Fusarium and Stachybotrys genera. The aim of the study was to investigate the effect of mycotoxins on the occurrence of apoptosis in the swine kidney (SK) epithelial cells. For this purpose, trichothecene T2 toxin was used at a concentration of 2.5 µM and 25 µM, aflatoxin B1 at a dose of 10 µM and 30 µM, and ochratoxin A concentrations of 50 µM and 80 µM. The results were assessed using flow cytometer Muse Cell Analyzer (Merck). Studies have shown high sensitivity of the cell line SK on mycotoxins. Apoptosis was caused by all kinds of toxins and depended on the dose of examined substance. T2 toxin at a concentration of 2.5 µM caused apoptosis in 6.9% of the cells, whereas at a concentration of 25 µM in 26.35% of the cells. Aflatoxin B1 used at concentrations of 10 µM and 30 µM caused apoptosis in 13.5% and 40.6% of the cells, respectively. The use of ochratoxin A in concentrations of 50 µM and 80 µM caused the occurrence of apoptosis respectively in 68.51% and 60.41% of the cells.
3
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Occurrence of fungi and cytotoxicity of the species: Aspergillus ochraceus, Aspergillus niger and Aspergillus flavus isolated from the air of hospital wards

81%
Gniadek A., Krzyściak P., Twarużek M., Macura A. B.
International Journal of Occupational Medicine and Environmental Health
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2017
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vol. 30
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issue 2
231-239
EN
Objectives The basic care requirement for patients with weakened immune systems is to create the environment where the risk of mycosis is reduced to a minimum. Material and Methods Between 2007 and 2013 air samples were collected from various wards of a number of hospitals in Kraków, Poland, by means of the collision method using MAS-100 Iso MH Microbial Air Sampler (Merck Millipore, Germany). The air mycobiota contained several species of fungi, and almost 1/3 of it was made up of the species of the Aspergillus genus. Sixty-one strains of species other than A. fumigatus were selected for the research purposes, namely: 28 strains of A. ochraceus, 22 strains of A. niger and 11 strains of A. flavus species. Selected fungi underwent a cytotoxicity evaluation with the application of the MTT colorimetric assay (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide). The assay assesses cell viability by means of reducing the yellow tetrazolium salt to insoluble formazan. A semi-quantitative scale for cytotoxicity grading was adopted: low cytotoxic effect (+) with half maximal inhibitory concentration (IC₅₀) for values ranging from 31.251 cm²/ml to 7.813 cm²/ml, medium cytotoxic effect (++) for values ranging from 3.906 cm²/ml to 0.977 cm²/ml and the high one (+++) for values ranging from 0.488 cm²/ml to 0.061 cm²/ml. The absence of cytotoxicity was determined when the IC₅₀ values was at ≥ 50. Results For 48 samples the analyzed fungi displayed the cytotoxic effect with A. ochraceus in 26 out of 28 cases, with 11 strains displaying the high cytotoxic effect. The lowest cytotoxicity was displayed by fungi of A. niger in 13 out of 22 cases, and the major fungi of A. flavus species were toxic (9 out of 11 cases). Conclusions A half of the fungi displayed the low cytotoxic effect. On the basis of the comparison of average cytotoxicity levels it was determined that there were significant differences in the levels of cytotoxicity of the analyzed fungi. However, such statement may not provide grounds for a definite conclusion about the compared species of fungi that display a more cytotoxic effect than others. Int J Occup Med Environ Health 2017;30(2):231–239
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